Fully automated production of calibration standards

The production of calibration standards in analytical laboratories is very time-consuming. Standards must be prepared precisely and documented accurately. The CHRONECT Workstation MultiMix achieves this while complying with the SANTE guidelines.


Requirements of SANTE 12682/2019

The SANTE document essentially sets out four requirements for the production of calibration standards:

  • Reference substances and stock solutions should be stored in a cool place, preferably in a freezer, excluding light and humidity.
  • The documentation created during production should ensure full traceability of all steps.
  • The standards should be permanently marked and should be remixed after heating to room temperature.
  • If septa are punctured during standard production, they should be replaced as soon as possible.

Implementation with the CHRONECT Workstation MultiMix

The workstation is based on the modular system of the CHRONECT Robotic Autosampler, which is optimally utilized by the CHRONOS software:

Peltier-cooled drawer system
The aliquot of the stock solutions is stored in a Peltier-cooled drawer system, which is flushed with nitrogen to exclude moisture. By regularly closing the drawers, the standards are stored in the dark.

Documentation by barcodes
Traceability is ensured through the use of 2D barcodes and a software package. The barcode reader was developed by Axel Semrau and is fully integrated into the system. The software creates suitable labels for the stock solutions, which are printed on solvent-resistant foil by means of a barcode printer. Before each dosage, the barcode is used to check the correctness of the stock solution.

Mixing by Vortex-Mixer
Re-mixing after heating to room temperature is achieved by using a vortex mixer.

Protection of the septa
To prevent the septa from being punctured, the vial is opened with a DeCapper before dosing and closed again after collection.


Advantages of automated production of working mixes

  • Compliance with SANTE guidelines
  • Production independent of working hours through automation
  • Less production time than with manual production
  • Accuracy of the concentrations can be checked at any time through volumetric dosing and gravimetric precision
  • High accuracy
  • High reproducibility
  • Reliability through integrated controls
  • Transferable from pesticide analysis to other substance groups